BMRB Entry 10083
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10083
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Title: PDZ domain of human RIM2B
Deposition date: 2007-01-24 Original release date: 2008-08-14
Authors: Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation: Nagashima, T.; Hayashi, F.; Yokoyama, S.. "PDZ domain of human RIM2B" . ., .-..
Assembly members:
PDZ domain, polymer, 131 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
PDZ domain: GSSGSSGHSHSDKHPVTWQP
SKDGDRLIGRILLNKRLKDG
SVPRDSGAMLGLKVVGGKMT
ESGRLCAFITKVKKGSLADT
VGHLRPGDEVLEWNGRLLQG
ATFEEVYNIILESKPEPQVE
LVVSRSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 561 |
| 15N chemical shifts | 136 |
| 1H chemical shifts | 899 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Regulating synaptic membrane exocytosis protein 2 | 1 |
Entities:
Entity 1, Regulating synaptic membrane exocytosis protein 2 131 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | HIS | SER | HIS | ||||
| 2 | SER | ASP | LYS | HIS | PRO | VAL | THR | TRP | GLN | PRO | ||||
| 3 | SER | LYS | ASP | GLY | ASP | ARG | LEU | ILE | GLY | ARG | ||||
| 4 | ILE | LEU | LEU | ASN | LYS | ARG | LEU | LYS | ASP | GLY | ||||
| 5 | SER | VAL | PRO | ARG | ASP | SER | GLY | ALA | MET | LEU | ||||
| 6 | GLY | LEU | LYS | VAL | VAL | GLY | GLY | LYS | MET | THR | ||||
| 7 | GLU | SER | GLY | ARG | LEU | CYS | ALA | PHE | ILE | THR | ||||
| 8 | LYS | VAL | LYS | LYS | GLY | SER | LEU | ALA | ASP | THR | ||||
| 9 | VAL | GLY | HIS | LEU | ARG | PRO | GLY | ASP | GLU | VAL | ||||
| 10 | LEU | GLU | TRP | ASN | GLY | ARG | LEU | LEU | GLN | GLY | ||||
| 11 | ALA | THR | PHE | GLU | GLU | VAL | TYR | ASN | ILE | ILE | ||||
| 12 | LEU | GLU | SER | LYS | PRO | GLU | PRO | GLN | VAL | GLU | ||||
| 13 | LEU | VAL | VAL | SER | ARG | SER | GLY | PRO | SER | SER | ||||
| 14 | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.17 mM; PiNa 20 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8992, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA34471 BAB18975 BAC98016 BAG10377 BAG54403 |
| EMBL | CAH18227 |
| GB | AAC19157 AAF81644 AAF81645 AAF81646 AAF81647 |
| REF | NP_001093587 NP_001243311 NP_001243312 NP_001269810 NP_055492 |
| SP | Q9EQZ7 Q9JIS1 Q9UQ26 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts