BMRB Entry 10081
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10081
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Title: Solution structure of the PDZ domain of mouse Rhophilin-2
Deposition date: 2007-01-23 Original release date: 2008-08-14
Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PDZ domain of mouse Rhophilin-2" . ., .-..
Assembly members:
PDZ domain, polymer, 111 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
PDZ domain: GSSGSSGSASKRWSPPRGIH
FTVEEGDLGFTLRGNTPVQV
HFLDPHCSASLAGAKEGDYI
VSIQGVDCKWLTVSEVMKLL
KSFGGEEVEMKVVSLLDSTS
SMHNKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 427 |
| 15N chemical shifts | 100 |
| 1H chemical shifts | 678 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rhophilin, Rho GTPase binding protein 2 | 1 |
Entities:
Entity 1, rhophilin, Rho GTPase binding protein 2 111 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | ALA | SER | ||||
| 2 | LYS | ARG | TRP | SER | PRO | PRO | ARG | GLY | ILE | HIS | ||||
| 3 | PHE | THR | VAL | GLU | GLU | GLY | ASP | LEU | GLY | PHE | ||||
| 4 | THR | LEU | ARG | GLY | ASN | THR | PRO | VAL | GLN | VAL | ||||
| 5 | HIS | PHE | LEU | ASP | PRO | HIS | CYS | SER | ALA | SER | ||||
| 6 | LEU | ALA | GLY | ALA | LYS | GLU | GLY | ASP | TYR | ILE | ||||
| 7 | VAL | SER | ILE | GLN | GLY | VAL | ASP | CYS | LYS | TRP | ||||
| 8 | LEU | THR | VAL | SER | GLU | VAL | MET | LYS | LEU | LEU | ||||
| 9 | LYS | SER | PHE | GLY | GLY | GLU | GLU | VAL | GLU | MET | ||||
| 10 | LYS | VAL | VAL | SER | LEU | LEU | ASP | SER | THR | SER | ||||
| 11 | SER | MET | HIS | ASN | LYS | SER | GLY | PRO | SER | SER | ||||
| 12 | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1 mM; acetate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 4.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Jhonson, B.A. - data analysis
Kujira v0.891, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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