BMRB Entry 10075
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10075
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Title: The fourth FN3 domain of human sidekick-2
Deposition date: 2006-12-25 Original release date: 2008-08-14
Authors: Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation: Nagashima, T.; Hayashi, F.; Yokoyama, S.. "The fourth FN3 domain of human sidekick-2" . ., .-..
Assembly members:
FN3 domain, polymer, 119 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
FN3 domain: GSSGSSGPQLVRTHEDVPGP
VGHLSFSEILDTSLKVSWQE
PGEKNGILTGYRISWEEYNR
TNTRVTHYLPNVTLEYRVTG
LTALTTYTIEVAAMTSKGQG
QVSASTISSGVPPSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 479 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 757 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | sidekick 2 | 1 |
Entities:
Entity 1, sidekick 2 119 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | GLN | LEU | ||||
| 2 | VAL | ARG | THR | HIS | GLU | ASP | VAL | PRO | GLY | PRO | ||||
| 3 | VAL | GLY | HIS | LEU | SER | PHE | SER | GLU | ILE | LEU | ||||
| 4 | ASP | THR | SER | LEU | LYS | VAL | SER | TRP | GLN | GLU | ||||
| 5 | PRO | GLY | GLU | LYS | ASN | GLY | ILE | LEU | THR | GLY | ||||
| 6 | TYR | ARG | ILE | SER | TRP | GLU | GLU | TYR | ASN | ARG | ||||
| 7 | THR | ASN | THR | ARG | VAL | THR | HIS | TYR | LEU | PRO | ||||
| 8 | ASN | VAL | THR | LEU | GLU | TYR | ARG | VAL | THR | GLY | ||||
| 9 | LEU | THR | ALA | LEU | THR | THR | TYR | THR | ILE | GLU | ||||
| 10 | VAL | ALA | ALA | MET | THR | SER | LYS | GLY | GLN | GLY | ||||
| 11 | GLN | VAL | SER | ALA | SER | THR | ILE | SER | SER | GLY | ||||
| 12 | VAL | PRO | PRO | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: FN3 domain, [U-13C; U-15N], 1.21 mM; d-TRIS 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8992, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 900 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB |
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