BMRB Entry 10059
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10059
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Title: Solution Structure of the Band 7 Domain of the mouse Flotillin 2 Protein
Deposition date: 2006-11-29 Original release date: 2008-08-13
Authors: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Band 7 Domain of the mouse Flotillin 2 Protein" . ., .-..
Assembly members:
Band 7 Domain, polymer, 143 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Band 7 Domain: GSSGSSGQRISLEIMTLQPR
CEDVETAEGVALTVTGVAQV
KIMTEKELLAVACEQFLGKN
VQDIKNVVLQTLEGHLRSIL
GTLTVEQIYQDRDQFAKLVR
EVAAPDVGRMGIEILSFTIK
DVYDKVDYLSSLGKTQTSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 607 |
| 15N chemical shifts | 148 |
| 1H chemical shifts | 999 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Flotillin 2 | 1 |
Entities:
Entity 1, Flotillin 2 143 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | ARG | ILE | ||||
| 2 | SER | LEU | GLU | ILE | MET | THR | LEU | GLN | PRO | ARG | ||||
| 3 | CYS | GLU | ASP | VAL | GLU | THR | ALA | GLU | GLY | VAL | ||||
| 4 | ALA | LEU | THR | VAL | THR | GLY | VAL | ALA | GLN | VAL | ||||
| 5 | LYS | ILE | MET | THR | GLU | LYS | GLU | LEU | LEU | ALA | ||||
| 6 | VAL | ALA | CYS | GLU | GLN | PHE | LEU | GLY | LYS | ASN | ||||
| 7 | VAL | GLN | ASP | ILE | LYS | ASN | VAL | VAL | LEU | GLN | ||||
| 8 | THR | LEU | GLU | GLY | HIS | LEU | ARG | SER | ILE | LEU | ||||
| 9 | GLY | THR | LEU | THR | VAL | GLU | GLN | ILE | TYR | GLN | ||||
| 10 | ASP | ARG | ASP | GLN | PHE | ALA | LYS | LEU | VAL | ARG | ||||
| 11 | GLU | VAL | ALA | ALA | PRO | ASP | VAL | GLY | ARG | MET | ||||
| 12 | GLY | ILE | GLU | ILE | LEU | SER | PHE | THR | ILE | LYS | ||||
| 13 | ASP | VAL | TYR | ASP | LYS | VAL | ASP | TYR | LEU | SER | ||||
| 14 | SER | LEU | GLY | LYS | THR | GLN | THR | SER | GLY | PRO | ||||
| 15 | SER | SER | GLY |
Samples:
sample_1: Band 7 Domain, [U-13C; U-15N], 1.02 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8996, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB23392 BAC26837 BAE28942 BAE41879 BAI46697 |
| EMBL | CAG46583 CAG47061 |
| GB | AAA65729 AAA93127 AAC98727 AAD00120 AAH03683 |
| REF | NP_001030543 NP_001035493 NP_001257729 NP_001257730 NP_001271156 |
| SP | A6QLR4 Q14254 Q60634 Q9Z2S9 |
| TPG | DAA18988 |
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