BMRB Entry 10034
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10034
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of Fibronectin type III domain derived from human KIAA0970 protein
Deposition date: 2006-11-01 Original release date: 2008-08-13
Authors: Kobayashi, N.; Koshiba, S.; Inoue, M.; Hayashi, F.; Kigawa, T.; Yokoyama, S.
Citation: Kobayashi, N.; Koshiba, S.; Inoue, M.; Hayashi, F.; Kigawa, T.; Yokoyama, S.. "Solution structure of Fibronectin type III domain derived from human KIAA0970 protein" . ., .-..
Assembly members:
Fibronectin type3 domain, polymer, 137 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Fibronectin type3 domain: GSSGSSGDEETKAFEALLSN
IVKPVASDIQARTVVLTWSP
PSSLINGETDESSVPELYGY
EVLISSTGKDGKYKSVYVGE
ETNITLNDLKPAMDYHAKVQ
AEYNSIKGTPSEAEIFTTLS
CEPDIPNPPRISGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 577 |
| 15N chemical shifts | 134 |
| 1H chemical shifts | 907 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KIAA0970 protein | 1 |
Entities:
Entity 1, KIAA0970 protein 137 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASP | GLU | GLU | ||||
| 2 | THR | LYS | ALA | PHE | GLU | ALA | LEU | LEU | SER | ASN | ||||
| 3 | ILE | VAL | LYS | PRO | VAL | ALA | SER | ASP | ILE | GLN | ||||
| 4 | ALA | ARG | THR | VAL | VAL | LEU | THR | TRP | SER | PRO | ||||
| 5 | PRO | SER | SER | LEU | ILE | ASN | GLY | GLU | THR | ASP | ||||
| 6 | GLU | SER | SER | VAL | PRO | GLU | LEU | TYR | GLY | TYR | ||||
| 7 | GLU | VAL | LEU | ILE | SER | SER | THR | GLY | LYS | ASP | ||||
| 8 | GLY | LYS | TYR | LYS | SER | VAL | TYR | VAL | GLY | GLU | ||||
| 9 | GLU | THR | ASN | ILE | THR | LEU | ASN | ASP | LEU | LYS | ||||
| 10 | PRO | ALA | MET | ASP | TYR | HIS | ALA | LYS | VAL | GLN | ||||
| 11 | ALA | GLU | TYR | ASN | SER | ILE | LYS | GLY | THR | PRO | ||||
| 12 | SER | GLU | ALA | GLU | ILE | PHE | THR | THR | LEU | SER | ||||
| 13 | CYS | GLU | PRO | ASP | ILE | PRO | ASN | PRO | PRO | ARG | ||||
| 14 | ILE | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: fibronectin type III domain, [U-13C; U-15N], 1.11 mM; Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.901, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian UNITY 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAA76814 BAG11267 BAG63723 |
| EMBL | CAG44602 CAG44603 CAH90829 CAI45989 |
| GB | AAI32813 AAI36618 AAI44302 EAX08803 EAX08804 |
| REF | NP_001073141 NP_001125472 NP_001265367 NP_055738 XP_001152472 |
| SP | Q5RBN1 Q9Y2H6 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts