BMRB Entry 10030
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10030
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Title: Solution structure of the forth CH domain from human plastin 3 T-isoform
Deposition date: 2006-11-01 Original release date: 2008-08-12
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the forth CH domain from human plastin 3 T-isoform" . ., .-..
Assembly members:
CH domain, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
CH domain: GSSGSSGNDDIIVNWVNRTL
SEAGKSTSIQSFKDKTISSS
LAVVDLIDAIQPGCINYDLV
KSGNLTEDDKHNNAKYAVSM
ARRIGARVYALPEDLVEVKP
KMVMTVFACLMGRGMKRVSG
PSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 520 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 827 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | T-plastin | 1 |
Entities:
Entity 1, T-plastin 124 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | ASP | ASP | ||||
| 2 | ILE | ILE | VAL | ASN | TRP | VAL | ASN | ARG | THR | LEU | ||||
| 3 | SER | GLU | ALA | GLY | LYS | SER | THR | SER | ILE | GLN | ||||
| 4 | SER | PHE | LYS | ASP | LYS | THR | ILE | SER | SER | SER | ||||
| 5 | LEU | ALA | VAL | VAL | ASP | LEU | ILE | ASP | ALA | ILE | ||||
| 6 | GLN | PRO | GLY | CYS | ILE | ASN | TYR | ASP | LEU | VAL | ||||
| 7 | LYS | SER | GLY | ASN | LEU | THR | GLU | ASP | ASP | LYS | ||||
| 8 | HIS | ASN | ASN | ALA | LYS | TYR | ALA | VAL | SER | MET | ||||
| 9 | ALA | ARG | ARG | ILE | GLY | ALA | ARG | VAL | TYR | ALA | ||||
| 10 | LEU | PRO | GLU | ASP | LEU | VAL | GLU | VAL | LYS | PRO | ||||
| 11 | LYS | MET | VAL | MET | THR | VAL | PHE | ALA | CYS | LEU | ||||
| 12 | MET | GLY | ARG | GLY | MET | LYS | ARG | VAL | SER | GLY | ||||
| 13 | PRO | SER | SER | GLY |
Samples:
sample_1: CH domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.901, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD96521 BAF83883 BAG35308 BAG57642 BAG57725 |
| GB | AAB02844 AAH08588 AAH39049 AAH56898 AAI50495 |
| REF | NP_001129497 NP_001165806 NP_001253150 NP_001269266 NP_001269267 |
| SP | P13797 |
Download simulated HSQC data in one of the following formats:
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or all simulated shifts
SPARKY: Backbone
or all simulated shifts