BMRB Entry 10014

Name: Assignments of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus
Published: 2006-11-21

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PDB ID: 2f87
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR10014
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Assignments of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus PubMed: 16838833

Deposition date: 2005-12-02 Original release date: 2006-11-21

Authors: Okada, Kiyoshi; Takahashi, Mari; Sakamoto, Taiichi; Nakamura, Kouji; Kanai, Akio; Kawai, Gota

Citation: Okada, Kiyoshi; Takahashi, Mari; Sakamoto, Taiichi; Kawai, Gota; Nakamura, Kouji; Kanai, Akio. "Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus" Nucleos. Nucleot. Nucl. 25, 383-395 (2006).

Assembly members:
pfHE6-12, polymer, 12 residues, Formula weight is not available

Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 186497 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Pyrococcus Pyrococcus furiosus

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
pfHE6-12: GGCUGAAGGGCC

Data sets:

assigned_chemical_shifts
- pfhe6

Data type	Count
1H chemical shifts	64

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	helix6 in SRP RNA	1

Entities:

Entity 1, helix6 in SRP RNA 12 residues - Formula weight is not available

1

G

C

U

G

A

G

2

C

Samples:

he6_1: pfHE6-12 2.0 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; phosphate buffer Na 20 ± 0.1 mM

condition_1: pH: 6.5; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY	he6_1	not available	condition_1
TOCSY	he6_1	not available	condition_1
1H-13C HSQC	he6_1	not available	condition_1
DQF-COSY	he6_1	not available	condition_1

Software:

xwinnmr v2.6 -

FELIX v97.0 -

DISCOVER v97.0 -

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz